Publications

@article{boreal:298422,

title = {Screening for Heavy Metal-Resistant Clones in the Xero-Halophyte Atriplex halimus L.: A Prerequisite for Phytoremediation of Polymetallic Mining Pollution in Arid Areas},

author = {Rania Zaghdoudi and Souhir Sghayar and Maher Necib and Paul Dekaezmaeker and Hélène Dailly and Walid Zorrig and Chedly Abdelly and Ahmed Debez and Stanley Lutts},

url = {http://hdl.handle.net/2078.1/298422},

doi = {10.1007/s41742-025-00738-2},

issn = {2008-2304},

year  = {2025},

date = {2025-01-01},

journal = {International Journal of Environmental Research},

volume = {19},

number = {3},

pages = {20},

publisher = {Springer Science and Business Media LLC},

abstract = {Atriplex halimus L. is a promising xero-halophyte species for phytoremediation purposes but displays high levels of genetic variability. As an attempt to select uniform material suitable for phytomanagement, five clones were established by cuttings from three non-polluted sites (Tunis, Nabeul and Sfax), one moderately Pb-polluted site (Sousse) and a highly polymetallic polluted mining site (Gafsa). Cuttings were cultivated during 90 days under controlled conditions on soil issued from the most polluted area. The clone from Gafsa accumulated higher concentrations of metals in roots (Cr) and leaves (Cd, Sr, Zn and Cu) than other clones. Gafsa showed the highest absorption efficiency, translocation factor, bioconcentration factor and bioaccumulation coefficient compared to other clones but displayed the lowest relative growth rate (RGR) value while the highest RGR was found in the clone from Sousse. Heavy metal tolerance in Gafsa was not related to a more efficient management of oxidative stress or higher concentration of phytochelatins. Total amount of Sr and Zn removed from the substrate was the highest for Sousse while removal of Cd, Cu, Cr and Ni was the highest for Gafsa. It is concluded that cuttings allow to obtain uniform material for phytoremediation by Atriplex halimus and that combining different clones with complementary properties is an attractive option for phytomanagement of polymetallic polluted soils by this species.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:300969,

title = {The sex of the mother plant affects the sex variation in the offspring in Dioscorea rotundata (Poir.)},

author = {Narcisse Denadi and Gabin Agban and Aline Vanhove and Elie Assaba and Petre I. Dobrev and Václav Motyka and Jeanne Zoundjihékpon and Christophe Gandonou and Muriel Quinet},

url = {http://hdl.handle.net/2078.1/300969},

doi = {10.1016/j.sajb.2025.04.033},

issn = {1727-9321},

year  = {2025},

date = {2025-01-01},

journal = {South African Journal of Botany},

volume = {181},

pages = {415-425},

publisher = {Elsevier BV},

address = {Amsterdam},

abstract = {In cultivated yams Dioscorea rotundata, spontaneous sex variation occurs in the offspring despite vegetative propagation. In this study, sex variation was investigated as a function of the region (head, middle, or base) of the seed-tuber and the sex of the mother plant. Tuber-seeds were sown from the head, middle, and base regions of tubers of 6 cultivars, and germination, flowering and sex of the offspring were studied. To determine a possible relationship between the hormone content of the mother tuber and sex variation, the phytohormone profile of the head, middle, and base regions was compared in tubers from male, female, and monoecious plants. Our results showed that the region of the seed-tuber affected germination, as the germination rate of seed-tubers from the head region was 20–50 % higher than that of the middle and base regions, and they germinated 10 days earlier. The region of the seed-tuber also affected the flowering rate, which was 10–20 % higher for seed-tubers from the head region than from the base region, but it did not affect the sex of the offspring. Sex variations in the offspring were more related to the sex of the parents. Male and female cultivars had stable offspring, while monoecious cultivars had high sex variation (80 %) in their offspring. The hormonal profile of the tubers suggested a relationship between hormones and sex identity. Jasmonates and salicylic acid were more concentrated in the tubers of male and monoecious plants, while abscisic acid types, cytokinins and benzoic acid were more concentrated in the tubers of female plants. Further work is needed to clarify the role of these hormones in the control of sex expression in cultivated yam D. rotundata.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:300156,

title = {The Combined Impact of Higher Temperatures and Water Deficit During Tuber Bulking Induces Contrasting Photosynthetic Performance},

author = {Andrea Ávila-Valdés and Muriel Quinet and Stanley Lutts and Juan Pablo Martínez and Alejandra Zúñiga-Feest and X. Carolina Lizana},

url = {http://hdl.handle.net/2078.1/300156},

doi = {10.1007/s11540-025-09856-y},

issn = {1871-4528},

year  = {2025},

date = {2025-01-01},

journal = {Potato Research},

pages = {20},

publisher = {Springer Netherlands},

address = {Dordrecht},

abstract = {In Chile, most potato crops are cultivated under rainfed systems, frequently facing temperature increases and water deficits during the growing season. This field study aimed to evaluate the physiological responses and genotypic variations between the Chilean native potato variety Chona Negra and commercial varieties (Karú INIA and Desiree) when exposed to moderately high temperatures (+ 5.0 to + 5.9 °C) for 40 days during the tuber bulking phase under different hydro- (or water availability) conditions. Four treatments were applied: (i) ambient temperature with rainfed conditions (T0H0), (ii) high temperature with rainfed conditions (T1H0), (iii) ambient temperature with irrigation (T0H1), and (iv) high temperature with irrigation (T1H1). The results showed that lower water availability and a moderate temperature increase (+ 5 °C) affected the leaf pigment content, photosynthetic performance and, chlorophyll fluorescence in both commercial and native potato genotypes. While moderately high temperatures and water deficits generally did not have stronger combined effects than each condition alone, our findings highlight the critical role of water availability in environments like southern Chile. Notably, the native potato, Chona Negra, exhibited enhanced photosynthetic performance under higher temperatures with irrigation, associated with increases in specific leaf area, stomatal conductance, photochemical quenching, and chlorophyll content. These findings suggest that Chona Negra may possess adaptive traits that improve its tolerance to heat stress when adequate water is available. In contrast, commercial varieties appeared more susceptible to limitations in photosynthetic activity under higher or prolonged periods of elevated temperatures.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:298486,

title = {Plant secondary metabolites against biotic stresses for sustainable crop protection},

author = {Tanzim Jahan and Md. Nurul Huda and Kaixuan Zhang and Yuqi He and Dili Lai and Namraj Dhami and Muriel Quinet and Md. Arfan Ali and Ivan Kreft and Sun-Hee Woo and Milen I. Georgiev and Alisdair R. Fernie and Meiliang Zhou},

url = {http://hdl.handle.net/2078.1/298486},

doi = {10.1016/j.biotechadv.2025.108520},

issn = {0734-9750},

year  = {2025},

date = {2025-01-01},

journal = {Biotechnology Advances},

volume = {79},

pages = {108520},

publisher = {Elsevier BV},

abstract = {Sustainable agriculture practices are indispensable for achieving a hunger-free world, especially as the global population continues to expand. Biotic stresses, such as pathogens, insects, and pests, severely threaten global food security and crop productivity. Traditional chemical pesticides, while effective, can lead to environmental degradation and increase pest resistance over time. Plant-derived natural products such as secondary metabolites like alkaloids, terpenoids, phenolics, and phytoalexins offer promising alternatives due to their ability to enhance plant immunity and inhibit pest activity. Recent advances in molecular biology and biotechnology have improved our understanding of how these natural compounds function at the cellular level, activating specific plant defense through complex biochemical pathways regulated by various transcription factors (TFs) such as MYB, WRKY, bHLH, bZIP, NAC, and AP2/ERF. Advancements in multi-omics approaches, including genomics, transcriptomics, proteomics, and metabolomics, have significantly improved the understanding of the regulatory networks that govern PSM synthesis. These integrative approaches have led to the discovery of novel insights into plant responses to biotic stresses, identifying key regulatory genes and pathways involved in plant defense. Advanced technologies like CRISPR/Cas9-mediated gene editing allow precise manipulation of PSM pathways, further enhancing plant resistance. Understanding the complex interaction between PSMs, TFs, and biotic stress responses not only advances our knowledge of plant biology but also provides feasible strategies for developing crops with improved resistance to pests and diseases, contributing to sustainable agriculture and food security. This review emphasizes the crucial role of PSMs, their biosynthetic pathways, the regulatory influence of TFs, and their potential applications in enhancing plant defense and sustainability. It also highlights the astounding potential of multi-omics approaches to discover gene functions and the metabolic engineering of genes associated with secondary metabolite biosynthesis. Taken together, this review provides new insights into research opportunities for enhancing biotic stress tolerance in crops through utilizing plant secondary metabolites.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:305605,

title = {Phylogenomics provides comprehensive insights into the evolutionary relationships among cultivated buckwheat species},

author = {Yaliang Shi and Bo Li and Yuanfen Gao and Xiaohan Wang and Yang Liu and Xiang Lu and Hao Lin and Wei Li and Muriel Quinet},

url = {http://hdl.handle.net/2078.1/305605},

doi = {10.1186/s13059-025-03793-2},

issn = {1474-760X},

year  = {2025},

date = {2025-01-01},

journal = {Genome Biology},

volume = {26},

number = {1},

pages = {31},

publisher = {BioMed Central Ltd.},

abstract = {Background Buckwheat belongs to the family Polygonaceae and genus Fagopyrum, which is characterized by high flavonoid content, short growth period, and strong environmental adaptability. Buckwheat has three cultivated species, including the annual food crops common buckwheat (Fagopyrum esculentum) and Tartary buckwheat (Fagopyrum tataricum), and the perennial traditional herbal medicine golden buckwheat (Fagopyrum cymosum). However, the unclear phylogenetic relationships among these three species based on genomic data limit buckwheat interspecific hybridization and genetic improvement. Results Despite their enormous differences in morphology and genome, we confirm the closet relationship between Fagopyrum cymosum and Fagopyrum tataricum, but not Fagopyrum esculentum. The results are also verified through collecting and sequencing an extensive sampling of cultivated/wild populations across all environmentally distinct regions in which these species are found. The changes in flowering time and style morphology controlled by the AP1 and S-ELF3 loci significantly contribute to the buckwheat speciation. The introgression from Fagopyrum cymosum into wild Fagopyrum tataricum explains why wild Fagopyrum tataricum exhibits seed morphology similar to Fagopyrum cymosum. Furthermore, the convergent traits of leaf morphology and higher flavonoid content between Fagopyrum cymosum and wild Fagopyrum esculentum are linked to high-altitude adaptation. Fagopyrum cymosum is more closely related to wild Fagopyrum tataricum, a fact that is confirmed by interspecific hybridization. Conclusions Our work provides a valuable example of how phylogenomics can be efficiently utilized for phylogenetic relationship analysis between crops and their wild species relatives, as well as elucidating the plant speciation from the perspectives of genomic evolution and adaptive mechanisms.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:299384,

title = {Integrative approaches to enhance reproductive resilience of crops for climate-proof agriculture},

author = {Muriel Quinet and Adrien Luyckx},

url = {http://hdl.handle.net/2078.1/299384},

doi = {10.1016/j.stress.2024.100704},

year  = {2025},

date = {2025-01-01},

journal = {Plant Stress},

volume = {15},

number = {/},

pages = {100704},

publisher = {Elsevier},

address = {Amsterdam},

abstract = {Worldwide agricultural systems are threatened by rising temperatures, extreme weather events, and shifting climate zones. Climate change-driven failure in sexual reproduction is a major cause for yield reduction in horticultural and grain crops. Consequently, understanding how climate change affects reproductive processes in crops is crucial for global food security and prosperity. The development of climate-proof crops, including maize, wheat, barley, rice, and tomato, requires new genetic material and novel management practices to ensure high productivity under less favorable conditions. Safeguarding successful plant reproduction is challenging due to the complex nature of this biological process, and therefore, a multifaceted approach is the key to success. In this review, we provide an overview of the processes underlying plant reproduction and how they are affected by different abiotic stresses related to climate change. We discuss how genetics, advanced breeding technologies, biotechnological innovations, and sustainable agronomic practices can collectively contribute to the development of resilient crop varieties. We also highlight the potential of artificial intelligence (AI) in optimizing breeding strategies, predicting climate impacts, and improving crop management practices to enhance reproductive resilience and ensure food security. Lastly, we discuss the vision of a new era in agriculture where diverse actors and stakeholders cooperate to create climate-proof crops.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:301424,

title = {Integrative approaches to enhance reproductive resilience of crops for climate-proof agriculture},

author = {Collins Agho and Adi Avni and Ariola Bacu and Salma Balazadeh and Shehzad Baloch and Muriel Quinet},

url = {http://hdl.handle.net/2078.1/301424},

doi = {10.1016/j.stress.2024.100704},

issn = {2667-064X},

year  = {2025},

date = {2025-01-01},

journal = {Plant Stress},

volume = {15},

pages = {100704},

publisher = {Elsevier Inc.},

abstract = {Worldwide agricultural systems are threatened by rising temperatures, extreme weather events, and shifting climate zones. Climate change-driven failure in sexual reproduction is a major cause for yield reduction in horticultural and grain crops. Consequently, understanding how climate change affects reproductive processes in crops is crucial for global food security and prosperity. The development of climate-proof crops, including maize, wheat, barley, rice, and tomato, requires new genetic material and novel management practices to ensure high productivity under less favorable conditions. Safeguarding successful plant reproduction is challenging due to the complex nature of this biological process, and therefore, a multifaceted approach is the key to success. In this review, we provide an overview of the processes underlying plant reproduction and how they are affected by different abiotic stresses related to climate change. We discuss how genetics, advanced breeding technologies, biotechnological innovations, and sustainable agronomic practices can collectively contribute to the development of resilient crop varieties. We also highlight the potential of artificial intelligence (AI) in optimizing breeding strategies, predicting climate impacts, and improving crop management practices to enhance reproductive resilience and ensure food security. Lastly, we discuss the vision of a new era in agriculture where diverse actors and stakeholders cooperate to create climate-proof crops.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:299476,

title = {Impact of Salinity on Sugar Composition and Partitioning in Relation to Flower Fertility in Solanum lycopersicum and Solanum chilense},

author = {Servane Bigot and Juan Pablo Martínez and Stanley Lutts and Muriel Quinet},

url = {http://hdl.handle.net/2078.1/299476},

doi = {10.3390/horticulturae11030285},

issn = {2311-7524},

year  = {2025},

date = {2025-01-01},

journal = {Horticulturae},

volume = {11},

number = {3},

pages = {285},

abstract = {Salinity negatively affects flower production and fertility in tomato but the underlying mechanisms are not fully understood. One hypothesis is that salinity affects sugar partitioning by reducing photosynthesis, which in turn affects source–sink relationships and hence the development of reproductive structures. This study investigates how salt stress alters sugar composition in leaves, flowers, and phloem sap of Solanum lycopersicum and its halophyte relative Solanum chilense, and how this may explain the effects on flower production and fertility. Salt stress increased flower abortion and reduced sepal length in S. lycopersicum, while decreasing pollen grain number in S. chilense. Photosynthetic nitrogen use efficiency was also reduced in S. lycopersicum. Salinity raised myo-inositol and sucrose concentrations in S. lycopersicum leaves but only slightly altered sugar concentrations in flowers. The concentration of sucrose in the foliar exudates was higher in S. chilense as compared to S. lycopersicum, suggesting a higher export of sucrose from the leaves. These findings suggest that S. lycopersicum maintains better metabolic function under salt stress, while S. chilense sustains sugar import to sink organs. Correlations between reproductive traits and sugar dynamics indicate that sugar distribution contributes to reproductive development under salinity stress.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:300158,

title = {Genome‐Wide Association Studies Reveal the Genetic Architecture of Ionomic Variation in Grains of Tartary Buckwheat},

author = {Zhirong Wang and Yuqi He and Mengyu Zhao and Xiang‐Qian Liu and Hao Lin and Yaliang Shi and Kaixuan Zhang and Guijie Lei and Dili Lai and Tong Liu and Xiaoyang Peng and Jiayue He and Wei Li and Xiangru Wang and Sun‐Hee Woo and Muriel Quinet and Alisdair R. Fernie and Xin‐Yuan Huang and Meiliang Zhou},

url = {http://hdl.handle.net/2078.1/300158},

doi = {10.1002/advs.202412291},

issn = {2198-3844},

year  = {2025},

date = {2025-01-01},

journal = {Advanced Science},

volume = {12},

publisher = {Wiley},

abstract = {Tartary buckwheat (Fagopyrum tataricum) is esteemed as a medicinal crop due to its high nutritional and health value. However, the genetic basis for the variations in Tartary buckwheat grain ionome remains inadequately understood. Through genome-wide association studies (GWAS) on grain ionome, 52 genetic loci are identified associated with 10 elements undergoing selection. Molecular experiments have shown that the variation in FtACA13’s promoter (an auto-inhibited Ca2+-ATPase) is accountable for grain sodium concentration and salt tolerance, which underwent selection during domestication. FtYPQ1 (a vacuolar amino acid transporter) exhibits zinc transport activity, enhancing tolerance to excessive zinc stress and raising zinc accumulation. Additionally, FtNHX2 (a Na+/H+ exchanger) positively regulates arsenic content. Further genomic comparative analysis of “20A1” (wild accession) and “Pinku” (cultivated accession) unveiled structural variants in key genes involved in ion uptake and transport that may result in considerable changes in their functions. This research establishes the initial comprehensive grain ionome atlas in Tartary buckwheat, which will significantly aid in genetic improvement for nutrient biofortification.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:297092,

title = {Exogenous melatonin enhances heat tolerance in buckwheat seedlings by modulating physiological response mechanisms},

author = {Zemiao Tian and Jiadong He and Zhanyu Wang and Qian Yang and Luping Ma and Yongzhi Qi and Jinbo Li and Yu Meng and Muriel Quinet},

url = {http://hdl.handle.net/2078.1/297092},

doi = {10.1016/j.plaphy.2025.109487},

issn = {0981-9428},

year  = {2025},

date = {2025-01-01},

journal = {Plant Physiology and Biochemistry},

volume = {220},

pages = {109487},

publisher = {Elsevier BV},

abstract = {Melatonin (MT) serves as a potent antioxidant in plant organisms, bolstering their resilience to temperature stress. In this study, the impact of MT on various buckwheat varieties under high-temperature stress conditions (40 °C) was investigated. Specifically, five buckwheat seedling varieties, comprising three sweet buckwheat variants (Fagopyrum esculentum) and two bitter buckwheat types (Fagopyrum tataricum), were subjected to foliar sprays of melatonin at concentrations of 50, 100 and 200 μM, with water at 25 °C employed as a control. Results demonstrated that exogenous MT at different concentrations improved the growth and physiological parameters of buckwheats, ameliorating damage induced by high-temperature stress. Notably, the application of 100 μM MT significantly augmented shoot biomasses of buckwheat seedlings under high-temperature conditions. Furthermore, the MT significantly increased the levels of osmotic adjustment substances and chlorophyll concentrations, enhanced antioxidant enzyme activities, chlorophyll fluorescence parameters, and improved photosynthetic gas exchange parameters in five different varieties of buckwheat. This led to the alleviation of damage to buckwheat seedlings subjected to high-temperature stress. Subsequently, five advanced statistical analysis methods: Principal Component Analysis, Grey Relational Analysis, Path Coefficient Analysis, Membership Function Method, and Coupling Coordination Analysis were employed to delve deeper into the existing data indicators. To summarize, the beneficial effect of exogenous melatonin on seedling growth is primarily achieved through the coordination and regulation of the antioxidant enzyme system and osmotic regulatory substances, ensuring the growth and development of buckwheat seedlings while also improving their heat tolerance. The treatment with a concentration of 100 μM of MT was the most effective.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:301119,

title = {Exogenous melatonin enhances drought tolerance and germination in common buckwheat seeds through the coordinated effects of antioxidant and osmotic regulation},

author = {Zemiao Tian and Jiadong He and Zhanyu Wang and Zhuo Zhang and Muriel Quinet and Yu Meng},

url = {http://hdl.handle.net/2078.1/301119},

doi = {10.1186/s12870-025-06632-5},

issn = {1471-2229},

year  = {2025},

date = {2025-01-01},

journal = {BMC Plant Biology},

volume = {25},

number = {1},

pages = {613},

publisher = {Springer Science and Business Media LLC},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:294629,

title = {Drought- and Salt-Tolerant Populations of the Xero-Halophyte Mediterranean Shrub Atriplex halimus L. Exhibit Contrasting Proline and Glycinebetaine Metabolism},

author = {Lydia Casasni and Cherifa Chaouia and Juan-Pablo Martínez and Muriel Quinet and Stanley Lutts},

url = {http://hdl.handle.net/2078.1/294629},

doi = {10.1007/s00344-024-11558-7},

issn = {1435-8107},

year  = {2025},

date = {2025-01-01},

journal = {Journal of Plant Growth Regulation},

volume = {44},

number = {5},

pages = {2445-2465},

publisher = {Springer New York LLC},

address = {New York},

abstract = {To determine the effects of ionic and nonionic osmotic stresses, seedlings from two populations of the xero-halophyte species Atriplex halimus L. Djelfa (salt-affected site) and Tamanrasset (non-saline arid area) were exposed for 10 days in nutrient solution to NaCl (100 or 300 mM) or to polyethylene glycol (PEG 6000; 50 or 100 g L− 1). Ions, proline and glycinebetaine were analyzed in relation to osmotic potential. Enzymes activities involved in proline or glycinebetaine synthesis were compared to the corresponding genes expression. Seedlings from Djelfa were more resistant to NaCl while those from Tamanrasset were more resistant to PEG. Salt-resistance in Djelfa was associated with greater Na+ accumulation and the maintenance of K+ concentration. Both ions significantly contributed to osmotic adjustment in salt-stress conditions. Proline accumulated mainly in response to nonionic water stress while glycinebetaine accumulated preferentially in response to salinity. Proline accumulation resulted from a stress-induced stimulation of Δ1-pyrroline-5-carboxylate reductase and ornithine δ-aminotransferase activities in Tamanrasset and from an inhibition of proline dehydrogenase activity in Djelfa. Glycinebetaine accumulation in Djelfa was associated to an increase in betaine aldehyde dehydrogenase activity while Tamarasset produced the glycinebetaine precursor choline in limiting amounts. Although genes coding for these enzymes were upregulated in most cases, there was a poor correlation between gene expression and enzyme activities suggesting important posttranscriptional regulation. The gene coding for proline/glycinebetaine transporter ProT was upregulated indicating a role of long-distance transport of osmotic compounds in plant resistance to environmental constraints.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:296730,

title = {Drought and High Temperatures Impact the Plant–Pollinator Interactions in Fagopyrum esculentum},

author = {Corentin Defalque and Joy Laeremans and Jonathan Drugmand and Chanceline Fopessi Tcheutchoua and Yu Meng and Meiliang Zhou and Kaixuan Zhang and Muriel Quinet},

url = {http://hdl.handle.net/2078.1/296730},

doi = {10.3390/plants14010131},

issn = {2223-7747},

year  = {2025},

date = {2025-01-01},

journal = {Plants},

volume = {14},

number = {1},

pages = {131},

publisher = {MDPI AG},

abstract = {As a result of climate change, temperate regions are facing the simultaneous increase in water and heat stress. These changes may affect the interactions between plants and pollinators, which will have an impact on entomophilous crop yields. Here, we investigated the consequences of high temperatures and water stress on plant growth, floral biology, flower-reward production, and insect visitation of five varieties of common buckwheat (Fagopyrum esculentum), an entomophilous crop of growing interest for sustainable agriculture. The plants were grown under two temperature regimes (21 °C/19 °C and 28 °C/26 °C, day/night) and two watering regimes (well-watered and water-stressed). Our results showed that the reproductive growth was more affected by drought and high temperatures than was the vegetative growth, and that combined stress had more detrimental effects. However, the impact of drought and high temperatures was variety-dependent. Drought and/or high temperatures reduced the number of open flowers per plant, as well as the floral resources (nectar and pollen), resulting in a decrease in pollinator visits, mainly under combined stress. Although the proportion of Hymenoptera visiting the flowers decreased with high temperatures, the proportion of Diptera remained stable. The insect visiting behavior was not strongly affected by drought and high temperatures. In conclusion, the modification of floral display and floral resources induced by abiotic stresses related to climate change alters plant–pollinator interactions in common buckwheat.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:298565,

title = {Buckwheat UDP‐Glycosyltransferase FtUGT71K6 and FtUGT71K7 Tandem Repeats Contribute to Drought Tolerance by Regulating Epicatechin Synthesis},

author = {Yuanfen Gao and Yaliang Shi and Tanzim Jahan and Md. Nurul Huda and Lin Hao and Yuqi He and Muriel Quinet and Hui Chen and Kaixuan Zhang and Meiliang Zhou},

url = {http://hdl.handle.net/2078.1/298565},

doi = {10.1111/pce.15412},

issn = {1365-3040},

year  = {2025},

date = {2025-01-01},

urldate = {2025-01-01},

journal = {Plant, Cell and Environment},

pages = {17},

publisher = {Wiley-Blackwell Publishing Ltd.},

address = {Chichester},

abstract = {Glycosyltransferase genes are organised as tandem repeats in the buckwheat genome, yet the functional implications and evolutionary significance of duplicated genes remain largely unexplored. In this study, gene family analysis revealed that FtUGT71K6 and FtUGT71K7 are tandem repeats in the buckwheat genome. Moreover, GWAS results for epicatechin suggested that this tandem repeat function was associated with epicatechin content of Tartary buckwheat germplasm, highlighting variations in the promoter haplotypes of FtUGT71K7 influenced epicatechin levels. FtUGT71K6 and FtUGT71K7 were shown to catalyse UDP‐glucose conjugation to cyanidin and epicatechin. Furthermore, overexpression of FtUGT71K6 and FtUGT71K7 increased total antioxidant capacity and altered metabolite content of the epicatechin biosynthesis pathway, contributing to improved drought tolerance, while overexpression of FtUGT71K6 significantly improved salt stress tolerance. However, overexpression of these two genes did not contribute to resistance against Rhizoctonia solani. Evolutionary selection pressure analysis suggested positive selection of a critical amino acid ASP‐53 in FtUGT71K6 and FtUGT71K7 during the duplication event. Overall, our study indicated that FtUGT71K6 and FtUGT71K7 play crucial roles in drought stress tolerance via modulating epicatechin synthesis in buckwheat.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:294626,

title = {Solid vermicompost and liquid vermicompost leachate have contrasting impacts on cadmium, lead and zinc phytoextraction by the Syrian beancaper Zygophyllum fabago L.},

author = {Salima Benazzouk and Stanley Lutts},

url = {http://hdl.handle.net/2078.1/294626},

doi = {10.1007/s11104-024-07099-3},

issn = {1573-5036},

year  = {2024},

date = {2024-01-01},

journal = {Plant and Soil : international journal on plant-soil relationships},

pages = {23 p.},

publisher = {Springer Netherlands},

address = {Dordrecht},

abstract = {Background and aims Vermicompost is a valuable amendment for phytomanagement of heavy metals contaminated areas but its impact on plant physiology remains poorly documented. It is available in liquid or solid forms, but these forms were never compared for their influence on pollutant accumulation by the plant in relation to soil heavy metals bioavailabilities. Methods Solid vermicompost (SV) and liquid vermicompost leachate (LVL) were tested on spiked soils in the absence (control) or in the presence of either 10 mg.Kg−1 Cd, 250 mg.Kg−1 Zn or 500 mg.Kg−1 Pb and cultivated with Zygophyllum fabago L. in a column device allowing leachate recovery and analysis. Plant physiological parameters were determined during 10 weeks of culture. Results SV reduced soil heavy metals bioavailability, improved plant growth and photosynthesis and reduced heavy metals concentrations in all organs. In contrast, LVL increased heavy metal bioavailability and root growth but inhibited shoot growth. LVL decreased root heavy metals concentrations but increased them in the shoots. Both types of amendments increased the total amount of heavy metal removed by the plant. Among the removed Cd and Zn less than 5% were removed by leaching and SV contributed to reduce percolation. SV contributed to stress avoidance by reducing pollutant uptake while LVL contributed to stress tolerance through reinforcement of endogenous protecting mechanisms. Conclusions Both SV and LVL are beneficial amendments for phytostabilization and phytoextraction of heavy metals by Z. fabago but act through distinct mechanisms on the plant in relation to mineral nutrition, oxidative stress and osmotic adjustment.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:294630,

title = {Season-dependent physiological behavior of Miscanthus x giganteus growing on heavy-metal contaminated areas in relation to soil properties},

author = {Stanley Lutts and Xiaoran Zhou and Flores-Bavestrello and Pierre Hainaut and Hélène Dailly and G. Debouche and Guy Foucart},

url = {http://hdl.handle.net/2078.1/294630},

doi = {10.1016/j.heliyon.2024.e25943},

issn = {2405-8440},

year  = {2024},

date = {2024-01-01},

journal = {Heliyon},

volume = {10},

number = {4},

pages = {e25943},

publisher = {Elsevier Ltd.},

abstract = {Miscanthus x giganteus is often considered as a suitable plant species for phytomanagement of heavy metal polluted sites. Nevertheless, its physiological behavior in response to the level of metal toxicity throughout the growing season remains poorly documented. Miscanthus x giganteus was cultivated on three sites in Belgium (BSJ: non-polluted control; CAR: slightly contaminated; VM strongly polluted by Cd, Pb, Cu, Zn, Ni and As). The presence of Miscanthus improved soil biological parameters assessed by measurement of enzyme activity and basal soil respiration on the three considered sites, although to a lower level on VM site. Heavy metal accumulation in the shoot was already recorded in spring. It displayed a contrasting distribution in the summer leaves since heavy metals and As metalloid accumulated mainly in the older leaves of CAR plants while showing a uniform distribution among leaves of different ages in VM plants. Comparatively to plants growing on BSJ, net photosynthesis decreased in plants growing on CAR and VM sites. The recorded decrease was mainly related to stomatal factors in CAR plants (decrease in stomatal conductance and in Ci) but to non-stomatal factors such as decrease in carboxylation efficiency and non-photochemical quenching in VM plants. Stomata remained open in VM plants which presented lower instantaneous and intrinsic water use efficiencies than CAR and BSJ plants. High proportions of heavy metals accumulated in CAR plants were bound to the cell wall fraction while the soluble and organelle-rich fractions were proportionally higher in VM plants, leading to a decrease in cell viability and cell membrane damages. It is concluded that not only the intensity but also the nature of physiological responses in Miscanthus x giganteus may drastically differ depending on the pollution level.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:294627,

title = {Histochemical and gene expression changes in Cannabis sativa hypocotyls exposed to increasing concentrations of cadmium and zinc},

author = {Roberto Berni and Jean-Francois Hausman and Stanley Lutts and Gea Guerriero},

url = {http://hdl.handle.net/2078.1/294627},

doi = {10.1016/j.stress.2024.100668},

issn = {2667-064X},

year  = {2024},

date = {2024-01-01},

journal = {Plant Stress},

volume = {14},

pages = {100668},

publisher = {Elsevier BV},

abstract = {Hemp (Cannabis sativa L.) is a versatile crop that produces cellulosic bast fibres used in textiles and biocomposites. Is also finds use in phytoremediation, being a good candidate for the cultivation on marginal lands, such as those contaminated by heavy metals (HMs). HMs like cadmium (Cd) and zinc (Zn) are known to affect plant growth and impair the biosynthesis of cellulose and lignin at the cell wall level. Since cellulose is the major component in the gelatinous layer of bast fibres, HMs can impact the structure of hemp fibres and, consequently, their mechanical properties. This study investigates how varying concentrations of Cd and Zn in the soil affect the bast fibres of hemp plantlets. The chosen model is the hypocotyl, as it is ideal for studying bast fibre development: it exhibits a temporal separation between the elongation and thickening phases within a short period of approximately three weeks. C. sativa plantlets were grown for 20 days, and the hypocotyls sampled to perform histochemical observations, gene expression analysis, as well as to quantify biomass yield and Cd/Zn accumulation. Hemp plantlets grown in soils with the three highest Zn concentrations were smaller than the control group, whereas no decrease in size was observed under elevated Cd concentrations. However, at the highest Cd concentration, the root system exhibited enhanced development, accompanied by a significant increase in dry weight across all the concentrations tested. The quantification of Cd and Zn showed that the roots were the main organs accumulating HMs. Cd at the two highest concentrations decreased significantly the lumen area of bast fibres and increased their cell wall thickness. Zn decreased significantly the lumen area, but it did not impact the thickness of the cell wall at the highest concentration. Cd also increased the number of secondary fibres. Immunohistochemistry highlighted a different pattern of crystalline cellulose distribution with a signal that was less homogeneous in the presence of Cd and Zn. Gene expression analysis revealed changes in transcripts encoding cellulose synthases, fasciclin-like arabinogalactan proteins, class III peroxidases. The results obtained shed light on the molecular response and bast fibre histological changes occurring in young hemp plants exposed to Cd and Zn.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:294628,

title = {Eustress and Plants: A Synthesis with Prospects for Cannabis sativa Cultivation},

author = {Roberto Berni and Margaux Thiry and Jean-Francois Hausman and Stanley Lutts and Gea Guerriero},

url = {http://hdl.handle.net/2078.1/294628},

doi = {10.3390/horticulturae10020127},

issn = {2311-7524},

year  = {2024},

date = {2024-01-01},

journal = {Horticulturae},

volume = {10},

number = {2},

pages = {127},

abstract = {Cannabis sativa L. is a species of great economic value. It is a medicinal plant that produces several bioactive phytochemicals, and the stems of the industrial cultivars, commonly referred to as “hemp”, are sources of both cellulosic fibers and hurds used in textiles and bio-composites. Environmental stresses of biotic and abiotic nature affect plant development and metabolism and can, consequently, impact biomass yield and phytochemical content. Stress factors can be divided into eustressors and distressors; while the former stimulate a positive response in terms of growth, productivity, and resistance, the latter impair plant development. Eustressors are factors that, applied at low–moderate doses, can improve plant performance. Several studies have investigated different types of distress in C. sativa and evaluated the impact on biomass and phytochemicals, while less attention has been paid to the study of eustress. This review discusses the concept of plant eustress by referring to the recent literature and extrapolates it to applications in C. sativa cultivation. The data available on the response of C. sativa to exogenous factors are reviewed, and then, salinity eustress applied to hemp cultivation is taken as a proof-of-concept example. The knowledge developed on plant eustress and the results collected so far are discussed in light of future applications to improve the production of biomass and phytochemicals in plants of economic interest. Emphasis is placed on the potential use of eustress in conjunction with other factors shown to impact both the physiological response and metabolism of Cannabis, among which there are macronutrients and biofertilizers. Perspectives are also drawn with respect to applying the knowledge developed on the elicitation of whole plants to Cannabis cell suspension cultures, which provide a controlled, scalable, and season-independent platform to produce secondary metabolites.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:292485,

title = {Stands diversity and population structure of five ethnoveterinary woody species according to climatic zone and human disturbance in Benin (West Africa)},

author = {Esaïe Tchetan and Thierry Dehouegnon Houehanou and Abiodoun Pascal Olounlade and Erick Virgile Bertrand Azando and Muriel Quinet and Marcel Romuald Benjamin Houinato and Sylvie Mawule Hounzangbe-Adote and Joëlle Quetin-Leclercq and Fernand Ahokanou Gbaguidi},

url = {http://hdl.handle.net/2078.1/292485},

doi = {10.1007/s44353-024-00006-y},

issn = {3004-9784},

year  = {2024},

date = {2024-01-01},

journal = {Discover Conservation},

volume = {1},

number = {1},

pages = {9 [1-23]},

publisher = {Springer Science and Business Media LLC},

address = {(United States) New York, NY},

abstract = {Farmers continue to use medicinal plants, alone or in combination with veterinary medicines, to treat animal diseases, especially in developing countries. The study aimed to determine the effect of climatic zone and anthropic disturbance level on the diversity of the natural stand and structures of Morinda lucida Benth, Spondias mombin L., Terminalia leiocarpa (DC.) Baill, Crossopteryx febrifuga (Afzel. ex G. Don) Benth, and Vitex doniana Sweet, five plant species often used in ethnoveterinary medicine in Benin. A floristic inventory was conducted in 206 plots of 0.15 ha each, distributed across 6 forests/hunting areas as well as surrounding fields and fallow land. The data were analyzed using R software by calculating the importance value index and the population structural parameters (density, basal area, mean height, etc.). The results showed that the floristic composition of the habitats of the five target species varied by climatic zone and disturbance level. Morinda lucida, S. mombin, and V. doniana were available in stands undergoing heavy disturbance in the Guinean zone, whereas T. leiocarpa and C. febrifuga were more available in the Sudano-Guinean and Sudanian zones. Values of structural parameters of these species were higher in the Guinean zone than in the other two zones. Furthermore, the results of the diameter structure showed C-values ranging from 1 to 3.6, suggesting the predominance of young individuals. This suggests strong human pressure on the adults of studied plants. These results should be used to contribute to the sustainable use of these species. Recommendations are made for the sustainable use of these five species, especially T. leiocarpa and C. febrifuga, which were heavily exploited given their low availability in stands under high human disturbance.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:280778,

title = {Phenolic acids and flavonoids classes in Acacia arabica (Lam) Willd. Seedling during water stress and subsequent re-hydration},

author = {Nassima Lassouane and Fatiha Aïd and Muriel Quinet and Stanley Lutts},

url = {http://hdl.handle.net/2078.1/280778},

doi = {10.1007/s11104-023-06375-y},

issn = {1573-5036},

year  = {2024},

date = {2024-01-01},

journal = {Plant and Soil : international journal on plant-soil relationships},

pages = {23},

publisher = {Springer Netherlands},

address = {Dordrecht},

abstract = {Background and aims A greenhouse experiment was conducted to analyze the physiological behavior of Acacia arabica in relation to concentration of phenolic compounds during drought and subsequent recovery. Methods Seedlings were exposed to water shortage during 32 days and then rehydrated and allowed to recover during 15 days. Parameters related to plant water status, photosynthesis and oxidative stress were quantified after 7, 15 and 32 days of stress, and after 7 and 15 days of recovery. Phenolic acids, flavonoids and enzyme activities involved in their synthesis were quantified. Results 88% of plants remained alive during the stress period and 10% died during recovery. Drought reduced water and osmotic potentials, stomatal conductance, net photosynthesis, instantaneous transpiration, non-photochemical quenching and flavanols concentration and increased proline, malondialdehyde, phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) activities, DPPH scavenging, hydroxycinnamic acids, hydroxybenzoic acids and flavonols concentration. Genes coding for PAL and chalcone synthase (CHS) were slightly downregulated at the end of drought while gene coding for C4H was upregulated at the beginning of drought. Parameters related to oxidative stress as well as hydroxybenzoic acids and flavonols remained at high values throughout the recovery period. Genes coding for C4H and CHS were up-regulated during recovery. Conclusions Acacia arabica is resistant to water stress at the seedling stage and able to recover after stress relief. Recovery induces a specific physiological status in terms of transpiration rates and management of oxidative stress in relation to flavonols and hydroxybenzoic acids concentrations.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:282676,

title = {Loss of function mutations at the tomato SSI2 locus impair plant growth and development by altering the fatty acid desaturation 

pathway},

author = {A. S. Quevedo‐Colmena and A. Ortiz‐Atienza and M. Jáquez‐Gutiérrez and Muriel Quinet and A. Atarés and F. J. Yuste‐Lisbona and V. Moreno and T. Angosto and R. Lozano},

url = {http://hdl.handle.net/2078.1/282676},

doi = {10.1111/plb.13591},

issn = {1438-8677},

year  = {2024},

date = {2024-01-01},

journal = {Plant Biology},

volume = {26},

number = {1},

pages = {106-116},

publisher = {Wiley},

abstract = {The stearoyl-ACP desaturase (SACPD) is a key enzyme in the regulation of saturated to unsaturated fatty acid ratio, playing a crucial role in regulating membrane stability and fluidity, as well as photosynthesis efficiency, which makes it an important research focus in crop species. • This study reports the characterization and molecular cloning of pale dwarf (pad), a new tomato (Solanum lycopersicum L.) T-DNA recessive mutant, which exhibits a dwarf and chlorotic phenotype. Functional studies of the T-DNA tagged gene were conducted, including phylogenetic analysis, expression and metabolomic analyses, and generation of CRISPR/Cas9 knockout lines.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:290649,

title = {Improving the Salt Tolerance of “Old Limachino Tomato” by Using a New Salt-Tolerant Rootstock},

author = {Juan-Pablo Martínez and Raúl Fuentes and Danitza Badilla and Camila Rosales and Juan Felipe Alfaro-Quezada and Francisco Correa and Carolina Lizana and Boris Sagredo and Muriel Quinet and Stanley Lutts},

url = {http://hdl.handle.net/2078.1/290649},

doi = {10.3390/horticulturae10080780},

issn = {2311-7524},

year  = {2024},

date = {2024-01-01},

journal = {Horticulturae},

volume = {10},

number = {8},

pages = {780},

publisher = {MDPI AG},

abstract = {Salinity is a major constraint limiting the yield of tomatoes. However, grafting strategies may help to overcome the salt toxicity of this important horticultural species if appropriate rootstocks are identified. The present study aimed to test a new rootstock, JUPAFORT1, obtained by crossing the glycophyte Solanum lycopersicum (cv. Poncho Negro) with the halophyte wild-related species Solanum chilense to improve the salinity tolerance of the Chilean tomato landrace Old Limachino Tomato (OLT). Intact OLT plants were exposed to 0, 80, or 160 mM of NaCl for 21 days at the vegetative stage and compared with self-grafted (L/L) and Limachino plants grafted on JUPAFORT1 rootstock (L/R) under a completely randomized design. JUPAFORT1 increased OLT scion vigor in the absence of salt but did not significantly increase fresh weight under stress conditions. However, JUPAFORT1 confers to the scion an anisohydric behavior contrasting with the isohydric behavior of L and L/L plants as indicated by measurements of stomatal conductance; L/R plants were able to maintain their metabolic status despite a slight decrease in the leaf’s relative water content. JUPAFORT1 rootstock also enabled the maintenance of photosynthetic pigment concentrations in the scion in contrast to L and L/L plants, which exhibited a decrease in photosynthetic pigments under stress conditions. L/R plants encountered oxidative stress at the highest stress intensity (160 mM of NaCl) only, while L and L/L plants suffered from oxidative damage at a lower dose (80 mM of NaCl). L/R plants behaved as includer plants and did not sequester Na+ in the root system, in contrast to L and L/L, which behaved as excluder plants retaining Na+ in the root system to avoid its translocation to the shoots. The expression of genes coding for ion transporters (HKT1.1, HKT1.2, LKT1, SKOR, SOS2, and SOS3) in the root system was not modified by salinity in L/R. In contrast, their expression varied in response to salinity in L and L/L. Overall, L/R plants exhibited higher physiological stability than L/L or L plants in response to an increasing NaCl dose and did not require additional energy investment to trigger an adaptative response to salinity. This suggests that the constitutive salinity tolerance of the halophyte S. chilense was maintained in the interspecific rootstock. JUPAFORT1 issued from S. lycopersicum x S. chilense may thus improve salt-stress resilience in OLT tomatoes. Additional studies are required to identify the molecular components involved in the root-to-shoot signaling pathway in this promising material.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:285117,

title = {Global nutritional challenges and opportunities: Buckwheat, a potential bridge between nutrient deficiency and food security},

author = {Rintu Jha and Kaixuan Zhang and Yuqi He and Nóra Mendler-Drienyovszki and Katalin Magyar-Tábori and Muriel Quinet and Mateja Germ and Ivan Kreft and Vladimir Meglič and Kiyokazu Ikeda and Mark A. Chapman and Dagmar Janovská and Grażyna Podolska and Sun-Hee Woo and Studer Bruno},

url = {http://hdl.handle.net/2078.1/285117},

doi = {10.1016/j.tifs.2024.104365},

issn = {1879-3053},

year  = {2024},

date = {2024-01-01},

journal = {Trends in Food Science & Technology},

volume = {145},

pages = {104365},

publisher = {Pergamon},

address = {Kidlington},

abstract = {Background The ability to ensure nutritional and food security is seriously threatened by the ever-growing global population. Overreliance on a few staple food crops such as wheat, rice and maize will no longer be able to satisfy the rising demand for future food. Even with increasing agricultural production, over 820 million people are still facing food insecurity and at least 2 billion are facing nutrition insecurity. Therefore, it is imperative to focus on finding simple and sustainable solution to the present threat of global food insecurity. Besides staple food crops, harnessing nutrient-dense, climate-resilient, and locally available crops for production, marketing and consumption as “Smart Foods” for the future is needed. Buckwheat is a potential smart food because of its stress resistance and nutritional properties as well as strong antioxidant activity due to presence of flavonoids. Scope and approach This paper comprises a comprehensive review of the scientific literature on the valorization of buckwheat crops in terms of germplasm resources, breeding and genetic improvements for yield, bioactive compounds, biological activities and its potential application in food and pharmaceutical industries. Key findings and conclusions Identification of elite cultivars and exploitation of buckwheat derived gluten-free bakery and non-bakery food stuffs and beverages such as tea, beer and wine should be explored for human consumption. Abundance of bioactive compounds results in high anti-oxidant, anti-microbial, anti-glycemic, anti-cancer properties and other health-promoting activities highlighting buckwheat's potential application as nutraceuticals and pharmaceuticals.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:285726,

title = {Genomic insight into the origin, domestication, dispersal, diversification and human selection of Tartary buckwheat},

author = {Yuqi He and Kaixuan Zhang and Yaliang Shi and Hao Lin and Xu Huang and Xiang Lu and Zhirong Wang and Xibo Feng and Muriel Quinet},

url = {http://hdl.handle.net/2078.1/285726},

doi = {10.1186/s13059-024-03203-z},

issn = {1474-760X},

year  = {2024},

date = {2024-01-01},

journal = {Genome Biology},

volume = {25},

number = {1},

pages = {24},

publisher = {BioMed Central Ltd.},

abstract = {Background Tartary buckwheat, Fagopyrum tataricum, is a pseudocereal crop with worldwide distribution and high nutritional value. However, the origin and domestication history of this crop remain to be elucidated. Results Here, by analyzing the population genomics of 567 accessions collected worldwide and reviewing historical documents, we find that Tartary buckwheat originated in the Himalayan region and then spread southwest possibly along with the migration of the Yi people, a minority in Southwestern China that has a long history of planting Tartary buckwheat. Along with the expansion of the Mongol Empire, Tartary buckwheat dispersed to Europe and ultimately to the rest of the world. The different natural growth environments resulted in adaptation, especially significant differences in salt tolerance between northern and southern Chinese Tartary buckwheat populations. By scanning for selective sweeps and using a genome-wide association study, we identify genes responsible for Tartary buckwheat domestication and differentiation, which we then experimentally validate. Comparative genomics and QTL analysis further shed light on the genetic foundation of the easily dehulled trait in a particular variety that was artificially selected by the Wa people, a minority group in Southwestern China known for cultivating Tartary buckwheat specifically for steaming as a staple food to prevent lysine deficiency. Conclusions This study provides both comprehensive insights into the origin and domestication of, and a foundation for molecular breeding for, Tartary buckwheat},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:286803,

title = {Exogenous melatonin improves germination rate in buckwheat under high temperature stress by regulating seed physiological and biochemical characteristics},

author = {Zemiao Tian and Mengyu Zhao and Junzhen Wang and Qian Yang and Yini Ma and Xinlei Yang and Luping Ma and Yongzhi Qi and Jinbo Li and Muriel Quinet and BaoSheng Shi and Yu Meng},

url = {http://hdl.handle.net/2078.1/286803},

doi = {10.7717/peerj.17136},

issn = {2167-8359},

year  = {2024},

date = {2024-01-01},

journal = {PeerJ},

volume = {12},

pages = {e17136},

publisher = {PeerJ, Ltd.},

address = {London},

abstract = {The germinations of three common buckwheat (Fagopyrum esculentum) varieties and two Tartary buckwheat (Fagopyrum tataricum) varieties seeds are known to be affected by high temperature. However, little is known about the physiological mechanism affecting germination and the effect of melatonin (MT) on buckwheat seed germination under high temperature. This work studied the effects of exogenous MT on buckwheat seed germination under high temperature. MT was sprayed. The parameters, including growth, and physiological factors, were examined. The results showed that exogenous MT significantly increased the germination rate (GR), germination potential (GP), radicle length (RL), and fresh weight (FW) of these buckwheat seeds under hightemperature stress and enhanced the content of osmotic adjustment substances and enzyme activity. Comprehensive analysis revealed that under high-temperature stress during germination, antioxidant enzymes play a predominant role, while osmotic adjustment substances work synergistically to reduce the extent of damage to the membrane structure, serving as the primary key indicators for studying high-temperature resistance. Consequently, our results showed that MT had a positive protective effect on buckwheat seeds exposed to high temperature stress, providing a theoretical basis for improving the ability to adapt to high temperature environments.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:292976,

title = {Evolution and Domestication of a Novel Biosynthetic Gene Cluster Contributing to the Flavonoid Metabolism and High‐Altitude Adaptability of Plants in the <i>Fagopyrum</i> Genus},

author = {Xu Huang and Yuqi He and Kaixuan Zhang and Yaliang Shi and Hui Zhao and Dili Lai and Hao Lin and Xiangru Wang and Zhimin Yang and Yawen Xiao and Wei Li and Yinan Ouyang and Sun Hee Woo and Muriel Quinet and Milen I. Georgiev and Alisdair R. Fernie and Xu Liu and Meiliang Zhou},

url = {http://hdl.handle.net/2078.1/292976},

doi = {10.1002/advs.202403603},

issn = {2198-3844},

year  = {2024},

date = {2024-01-01},

journal = {Advanced Science},

pages = {15},

publisher = {Wiley},

abstract = {The diversity of secondary metabolites is an important means for plants to cope with the complex and ever-changing terrestrial environment. Plant biosynthetic gene clusters (BGCs) are crucial for the biosynthesis of secondary metabolites. The domestication and evolution of BGCs and how they affect plant secondary metabolites biosynthesis and environmental adaptation are still not fully understood. Buckwheat exhibits strong resistance and abundant secondary metabolites, especially flavonoids, allowing it to thrive in harsh environments. A non-canonical BGC named UFGT3 cluster is identified, which comprises a phosphorylase kinase (PAK), two transcription factors (MADS1/2), and a glycosyltransferase (UFGT3), forming a complete molecular regulatory module involved in flavonoid biosynthesis. This cluster is selected during Tartary buckwheat domestication and is widely present in species of the Fagopyrum genus. In wild relatives of cultivated buckwheat, a gene encoding anthocyanin glycosyltransferase (AGT), which glycosylates pelargonidin into pelargonidin-3-O-glucoside, is found inserted into this cluster. The pelargonidin-3-O-glucoside can help plants resist UV stress, endowing wild relatives with stronger high-altitude adaptability. This study provides a new research paradigm for the evolutionary dynamics of plant BGCs, and offers new perspectives for exploring the mechanism of plant ecological adaptability driven by environmental stress through the synthesis of secondary metabolites.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:291875,

title = {Comprehensive assessment of fragrance profiles in peony flowers through HS-GC-IMS and metabolic pathway analysis},

author = {Yanhua Pan and Yini Ma and Jiadong He and Lu Zhang and Rong Huang and Ling Tang and Jiaxuan Ren and Weicheng Wang and Muriel Quinet and Yu Meng},

url = {http://hdl.handle.net/2078.1/291875},

doi = {10.1016/j.scienta.2024.113646},

issn = {0304-4238},

year  = {2024},

date = {2024-01-01},

journal = {Scientia Horticulturae},

volume = {338},

pages = {113646},

publisher = {Elsevier BV},

address = {Amsterdam},

abstract = {This study addresses the identification and comparison of volatile organic compounds (VOCs) in different peony varieties to elucidate their distinct aroma profiles and the common VOCs that constitute the unique fragrance of peony flowers, laying a foundation for breeding high-quality peony varieties and developing related products. Headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) combined with principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) was employed to analyze and differentiate the VOCs of eight peony varieties: TAOXI, ZIHELIAN, BAITIANE, YAOHUANG, DAFUGUI, DAOJIN, HONGXIUQIU, and MANAOPAN. The study identified 87 VOCs, revealing significant differences in VOC compositions among the samples. Notably, BAITIANE displayed distinct differences in both color and VOC composition compared to the other seven varieties. It exhibited uniquely high levels of butanoic acid-M, butanoic acid-D, hexanal-D, ethanol-D, and ethyl acetate. Twelve key differential VOCs were identified as having significant impacts: ethanol-D, hexanal-D, (e)-2-hexenal-D, (e)-2-hexenal-M, hexanal-M, butanoic acid-M, acetic acid-D, acetic acid-M, butanoic acid-D, ethyl acetate, thiophene, and dimethyl sulfide. KEGG pathway analysis highlighted the roles of xenobiotics biodegradation, terpenoid metabolism, and amino acid metabolism in VOC biosynthesis. The findings are significant for the breeding of fragrant peony varieties and the development of floral-based products. The findings provide theoretical support for breeding new horticultural flower varieties and developing commercial peony products, enhancing the understanding of floral scent chemistry, and offering valuable information for the perfumery, floral food processing, and related industries.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:296731,

title = {6-Benzylaminopurine (BAP) and Methyl Jasmonate (MeJa) Affect Sex Expression, Flowering Time and Flowering Intensity in Cultivated Yam Dioscorea rotundata (Poir.)},

author = {Narcisse Denadi and Aline Vanhove and Jeanne Zoundjihékpon and Christophe Gandonou and Muriel Quinet},

url = {http://hdl.handle.net/2078.1/296731},

doi = {10.3390/applbiosci3040035},

issn = {2813-0464},

year  = {2024},

date = {2024-01-01},

journal = {Applied Biosciences},

volume = {3},

number = {4},

pages = {559-576},

publisher = {MDPI AG},

abstract = {Cultivated yam (Dioscorea rotundata) is a staple tuber crop in West Africa which is mainly vegetatively propagated. Although the majority of yam cultivars flower, the control of their sexual reproduction remains largely unknown despite its importance for plant-breeding programs. While described as a dioecious species, yam has several monoecious (mix) cultivars that are often subject to spontaneous sex changes. This study aims to evaluate the impact of phytohormones on sex expression and flower development in cultivated yams D. rotundata. Exogenous applications of 1 mM 6-benzylaminopurine (BAP) and 1 mM methyl jasmonate (MeJa) were performed on seedlings of female (Gnidou, Anago), male (Flou) and mix (Katala and Laboko) cultivars. The mix cultivars produced monoecious, male and female plants while the female and male cultivars had rather stable sex. Our results showed that MeJa exhibited a significant masculinising effect in mix cultivars and induced inflorescence and flower malformations in female cultivars (56% in Gnidou and 37% in Anago). Most malformations were inflorescence branching and sterile flowers (non-differentiated ovary) with extra cycles of sepals. Moreover, MeJa reduced flowering time in the cultivars of all sexes and increased the number of inflorescences per plant as well as the number of flowers per plant. Our results showed that BAP reduced the flowering time, synchronized flowering in female plants and increased the number of inflorescences per plant in monoecious plants. However, our results did not allow for strong conclusions regarding the effect of BAP on sex expression due to the high proportion of female flowering in both the control and BAP-sprayed plants. Nevertheless, we did not observe any masculinising effect for BAP. Further research that would highlight hormone and homeotic gene interactions in flowering could be of key interest in understanding the hormonal control of sex in cultivated yams D. rotundata.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:275864,

title = {Nitrogen Use Efficiency of Rice Cultivars (Oryza sativa L.) Under Salt Stress and Low Nitrogen Conditions},

author = {Nhung Thi Hong Phan and Adrien Heymans and Mathieu Bonnave and Stanley Lutts and Cuong Van Pham and Pierre Bertin},

url = {http://hdl.handle.net/2078.1/275864},

doi = {10.1007/s00344-022-10660-y},

issn = {1435-8107},

year  = {2023},

date = {2023-01-01},

journal = {Journal of Plant Growth Regulation},

volume = {42},

number = {3},

pages = {1789-1803},

publisher = {Springer New York LLC},

address = {New York},

abstract = {Improving nitrogen use efficiency (NUE) under salt stress has become crucial for rice as it is increasingly facing two major environmental constraints: excessive nitrogen fertilization and soil salinization. However, the interaction between salinity and N levels is very complex and has not yet been considered from the perspective of reduced nitrogen input. We conducted a hydroponic experiment at the early tillering stage on the Yoshida solution to evaluate the impact of rising NaCl and decreasing N application on NUE of four rice cultivars cultivated under three NaCl (0, 56, and 113 mM) and four N (2.86, 1.43, 0.72, and 0.36 mM) concentrations. After 4 weeks, physiological NUE (pNUE), absorption NUE (aNUE), agronomical NUE (agNUE), N transport efficiency (NTE), and physiological traits were evaluated. Significant interactions between N and NaCl-applied concentrations were found in all measured parameters. In all cultivars, increasing the NaCl-applied concentration markedly decreased aNUE and agNUE. For each NaCl treatment, lowering the N applied sharply increased aNUE and agNUE, and this effect was stronger when the NaCl applied was higher. The effect of N lowering on pNUE depended on the NaCl treatment: it enhanced pNUE in the absence of NaCl but had no influence under the highest NaCl-applied concentration. Cultivars largely differed in response to NaCl. The aNUE—but not pNUE—differed between salt-tolerant and salt-sensitive cultivars: aNUE markedly decreased with NaCl concentration in the most salt-sensitive cultivar, whereas it was the highest at the intermediate NaCl concentration in the most salt-tolerant cultivar, especially under low N levels. This finding suggests that under salt conditions, the use of salt-tolerant rice genotypes combined with reducing N level application is necessary to improve NUE. The study of NUE in rice should be focused on the improvement of aNUE with a strong emphasis on the salt tolerance of cultivars.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:275865,

title = {Impact of salinity on yield-related parameters in two contrasting cultivars of \textit{Oryza glaberrima} Steud. in Benin},

author = {Hermann Prodjinoto and Christophe Gandonou and Willy Irakoze and Stanley Lutts},

url = {http://hdl.handle.net/2078.1/275865},

doi = {10.1017/s0014479723000030},

issn = {1469-4441},

year  = {2023},

date = {2023-01-01},

journal = {Experimental Agriculture},

volume = {59},

number = {59},

pages = {11 p.},

publisher = {Cambridge University Press},

address = {Cambridge},

abstract = {Salinity is one of the major environmental stresses limiting growth and yield of rice. The objective of the present study was to analyze the impact of NaCl on yield-related parameters of Oryza glaberrima. Two contrasted cultivars of Oryza glaberrima previously tested for salt resistance at the vegetative stage [salt-resistant (TOG5307) and salt-sensitive (TOG5949)] were irrigated with a saline solution containing 30 mM NaCl (EC: 3 dS.m−1 NaCl). After 6 months of treatments, mineral nutrient and yield-related parameters were assessed. Proline was quantified in the panicle leaf at the start of the grain filling stage. NaCl treatment affected most yield-related parameters: panicle length, panicle leaf dry weight, number of branches per panicle, panicle leaf length, days to 50% heading, straw fresh weight per plant, grain yield per plant, number of spikelets per panicle, and number of filled grains per panicle. The weight of 1,000 grains decreased in the salt-sensitive cultivar only, suggesting that grain filling processes were compromised. The salt-resistant cultivar TOG5307 was less affected than the salt-sensitive TOG5949 and accumulated lower amounts of Na+ in the grains. For both cultivars, hulls contained higher concentration of Na+ and K+ than grains. TOG5307 also contained more proline in the panicle leaf than TOG5949, suggesting that TOG5307 can cope with the osmotic component of salt stress. The cultivar exhibiting the highest salt resistance at the vegetative stage also exhibited the highest resistance at the reproductive one.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:275868,

title = {Effect of NaCl and EDDS on Heavy Metal Accumulation in Kosteletzkya pentacarpos in Polymetallic Polluted Soil},

author = {Mingxi Zhou and Zahar Kiamarsi and Ruiming Han and Mohammad Kafi and Stanley Lutts},

url = {http://hdl.handle.net/2078.1/275868},

doi = {10.3390/plants12081656},

issn = {2223-7747},

year  = {2023},

date = {2023-01-01},

journal = {Plants},

volume = {12},

number = {8},

pages = {1656},

publisher = {MDPI AG},

abstract = {The ability of plants to accumulate heavy metals is a crucial factor in phytoremediation. This study investigated the effect of NaCl and S,S-ethylenediaminesuccinic acid (EDDS) on heavy metal accumulation in Kosteletzkya pentacarpos in soil polluted with arsenic, cadmium, lead, and zinc. The addition of NaCl reduced the bioavailability of arsenic and cadmium, while EDDS increased the bioavailability of arsenic and zinc. The toxicity of the polymetallic pollutants inhibited plant growth and reproduction, but NaCl and EDDS had no significant positive effects. NaCl reduced the accumulation of all heavy metals in the roots, except for arsenic. In contrast, EDDS increased the accumulation of all heavy metals. NaCl reduced the accumulation of arsenic in both the main stem (MS) and lateral branch (LB), along with a decrease in cadmium in the leaves of the main stem (LMS) and zinc in the leaves of the lateral branch (LLB). Conversely, EDDS increased the accumulation of all four heavy metals in the LB, along with an increase in arsenic and cadmium in the LMS and LLB. Salinity significantly decreased the bioaccumulation factor (BF) of all four heavy metals, while EDDS significantly increased it. NaCl had different effects on heavy metals in terms of the translocation factor (TFc), increasing it for cadmium and decreasing it for arsenic and lead, with or without EDDS. EDDS reduced the accumulation of all heavy metals, except for zinc, in the presence of NaCl in polluted soil. The polymetallic pollutants also modified the cell wall constituents. NaCl increased the cellulose content in the MS and LB, whereas EDDS had little impact. In conclusion, salinity and EDDS have different effects on heavy metal bioaccumulation in K. pentacarpos, and this species has the potential to be a candidate for phytoremediation in saline environments.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:276975,

title = {Sodium and chloride accumulation and repartition differed between the cultivated tomato (Solanum lycopersicum) and its wild halophyte relative Solanum chilense under salt stress},

author = {Servane Bigot and Markéta Fuksová and Juan-Pablo Martínez and Stanley Lutts and Muriel Quinet},

url = {http://hdl.handle.net/2078.1/276975},

doi = {10.1016/j.scienta.2023.112324},

issn = {0304-4238},

year  = {2023},

date = {2023-01-01},

journal = {Scientia Horticulturae},

volume = {321},

pages = {112324},

publisher = {Elsevier BV},

address = {Amsterdam},

abstract = {Salinity is of growing global concern and affects the yield of several crop species, including tomato (Solanum lycopersicum). Halophyte wild relatives could be used to improve salt tolerance of crop species. Among wild tomato relatives, Solanum chilense was shown to be NaCl-tolerant but its strategy for Na+ accumulation and repartition remained poorly understood, and its Cl−accumulation was never investigated. In this study, both species were cultivated under salinity (0, 60 and 120 mM NaCl) during vegetative and reproductive phases. We investigated the ion (Na+, K+, Cl−) accumulation and repartition in the different organs (leaves, stems, roots, inflorescences), the expression of Na+ and Cl− transporters in vegetative organs and the role of these ions in osmotic management. Solanum chilense accumulated mainly Na+ in the shoots while S. lycopersicum accumulated it mainly in the roots so that the Na+ shoot/root ratio was 10 times higher in S. chilense than in S. lycopersicum. This suggests that S. chilense had an includer strategy while S. lycopersicum had an excluder strategy towards Na+. The excluder behavior of S. lycopersicum was linked to the high expression of HKT1;2 and SOS pathway genes in roots. In contrast to Na+, both species accumulated Cl− in a similar way and Cl− content was higher in the shoots than in the roots in both species. In addition, both species limited the entry of Na+ and Cl− in the inflorescences. The Na+ and Cl− concentrations were respectively about 3 and 2 times lower in the inflorescences than in the leaves. Ions also contributed to osmotic adjustment, mainly Cl− in S. lycopersicum and Na+ and K+ in S. chilense. Overall, our results highlight the salt-tolerance characteristics of S. chilense compared to the cultivated tomato.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:275588,

title = {Rootstock increases the physiological defence of tomato plants against Pseudomonas syringae pv. tomato infection},

author = {Juan Felipe Alfaro-Quezada and Juan Pablo Martínez and Sebastian Molinett and Miryam Valenzuela and Ivan Montenegro and Ingrid Ramírez and Fernando Dorta and Andrea Ávila-Valdés and Emna Gharbi and Mingxi Zhou and Hélène Dailly and Muriel Quinet and Stanley Lutts and Michael Seeger Pfeiffer and Wen-Ming Wang},

url = {http://hdl.handle.net/2078.1/275588},

doi = {10.1093/jxb/erad040},

issn = {1460-2431},

year  = {2023},

date = {2023-01-01},

journal = {Journal of Experimental Botany},

volume = {74},

number = {9},

pages = {2891-2911},

publisher = {Oxford University Press},

address = {Oxford},

abstract = {Climate change has intensified the infection of tomato plants by pathogens such as Pseudomonas syringae pv. tomato (Pst). Rootstocks may increase plant tolerance to leaf phytopathogens. The aim of this study was to evaluate the effects of the tolerant Poncho Negro (R) tomato rootstock on physiological defence and the role of hydrogen sulfide (H2S) in susceptible Limachino (L) tomato plant responses to Pst attack. Ungrafted (L), self-grafted (L/L), and grafted (L/R) plants were infected with Pst. Rootstock increased the concentration of antioxidant compounds including ascorbate in the scion. Tolerant rootstock induced an increase of H2S in the scion, which correlated with enhanced expression of the SlAPX2 gene. A high accumulation of salicylic acid was observed in Pst-inoculated grafted L/L and L/R plants, but this was higher in L/R plants. The increase of H2S during Pst infection was associated with a reduction of ethylene in L/R plants. Our study indicates that the Poncho Negro rootstock reduced the symptoms of bacterial speck disease in the Limachino tomato plants, conferring tolerance to Pst infection. This study provides new knowledge about the impact of rootstock in the defence of tomato plants against leaf pathogens that could be used in sustainable management of tomato cultivation.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:281715,

title = {Intra- and inter-specific reproductive barriers in the tomato clade},

author = {Pauline Moreels and Servane Bigot and Corentin Defalque and Francisco Correa Soto and Juan-Pablo Martinez and Stanley Lutts and Muriel Quinet},

url = {http://hdl.handle.net/2078.1/281715},

doi = {10.3389/fpls.2023.1326689},

issn = {1664-462X},

year  = {2023},

date = {2023-01-01},

journal = {Frontiers in Plant Science},

volume = {14},

pages = {12},

publisher = {Frontiers Research Foundation},

abstract = {Tomato (Solanum lycopersicum L.) domestication and later introduction into Europe resulted in a genetic bottleneck that reduced genetic variation. Crosses with other wild tomato species from the Lycopersicon clade can be used to increase genetic diversity and improve important agronomic traits such as stress tolerance. However, many species in the Lycopersicon clade have intraspecific and interspecific incompatibility, such as gametophytic self-incompatibility and unilateral incompatibility. In this review, we provide an overview of the known incompatibility barriers in Lycopersicon. We begin by addressing the general mechanisms self-incompatibility, as well as more specific mechanisms in the Rosaceae, Papaveraceae, and Solanaceae. Incompatibility in the Lycopersicon clade is discussed, including loss of self-incompatibility, species exhibiting only self-incompatibility and species presenting both self-compatibility and self-incompatibility. We summarize unilateral incompatibility in general and specifically in Lycopersicon, with details on the ’self-compatible x self-incompatible’ rule, implications of self-incompatibility in unilateral incompatibility and self-incompatibility-independent pathways of unilateral incompatibility. Finally, we discuss advances in the understanding of compatibility barriers and their implications for tomato breeding.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:275832,

title = {Comparison of the salt resistance of Solanum lycopersicum x Solanum chilense hybrids and their parents},

author = {Servane Bigot and Claire Leclef and Camila Rosales and Juan-Pablo Martinez and Stanley Lutts and Muriel Quinet},

url = {http://hdl.handle.net/2078.1/275832},

doi = {10.3389/fhort.2023.1130702},

issn = {2813-3595},

year  = {2023},

date = {2023-01-01},

journal = {Frontiers in Horticulture},

volume = {2},

pages = {1130702},

abstract = {Introduction: Soil salinization is a major global problem. Tomato (Solanum lycopersicum) is one of the main crops produced in the world and is cultivated in areas affected by salinity. However, it is considered as a glycophyte species and is sensitive to salt stress. Solanum chilense is a wild tomato native to the Atacama Desert and is therefore adapted to harsh environmental conditions, including salinity. It is considered as a promising source of genes for tomato improvement. However, the physiology of abiotic stress resistance remains largely unknown in S. chilense and no studies have investigated the resistance of S. lycopersicum x S. chilense interspecific hybrids and the potential value of their use in harsh environments. Methods: In this study, we compared the salt (0 vs. 100 mM NaCl) resistance of S. lycopersicum, S. chilense and their hybrids at vegetative and reproductive stages. Results: Overall, hybrids showed an intermediate behavior between their parents and a higher salt resistance than S. lycopersicum. Their physiological behavior toward salt stress was sometimes closed to S. lycopersicum and sometimes closed to S. chilense. Their sodium accumulation was intermediate between parents, with a sodium concentration closer to S. lycopersicum than S. chilense in roots, but with an aerial concentration closer to S. chilense than to S. lycopersicum. In inflorescences and fruits, the sodium concentration of hybrids was closer to S. lycopersicum than to S. chilense. Despite a decrease in instantaneous transpiration, the photosynthetic nitrogen use efficiency of hybrids was not decreased by salt stress: our results suggest a greater tolerance to the osmotic phase of salt stress in hybrids compared to the parents. Regarding the reproductive stage, inflorescence production and fruit quality were not affected by salt in hybrids. Discussion: This study highlights the potential use of hybrids in improving tomato for salt stress resistance.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:278296,

title = {Comparison of Salt Stress Tolerance among Two Leaf and Six Grain Cultivars of Amaranthus cruentus L.},

author = {Adrien Luyckx and Stanley Lutts and Muriel Quinet},

url = {http://hdl.handle.net/2078.1/278296},

doi = {10.3390/plants12183310},

issn = {2223-7747},

year  = {2023},

date = {2023-01-01},

journal = {Plants},

volume = {12},

number = {18},

pages = {3310},

publisher = {M D P I AG},

address = {Basel},

abstract = {Amaranths (Amaranthus L.) are multi-use crop species renowned for their nutritional quality and their tolerance to biotic and abiotic stresses. Since the soil salinity of croplands is a growing problem worldwide, we tested the salinity tolerance of six grain and two leaf cultivars of Amaranthus cruentus L. The plants were grown for 53 days under hydroponic conditions at 0, 50 and 100 mM NaCl. We investigated the growth rate, photosynthetic activity, mineral content, pigments and biochemical compounds involved in oxidative stress. Although 100 mM NaCl always decreased biomass production, we highlighted Don Leon and K91 as tolerant cultivars under moderate salt stress (50 mM NaCl). Under salinity, sodium accumulated more in the shoots than in the roots, particularly in the stems. Sodium accumulation in the plants decreased the net photosynthetic rate, transpiration rate and stomatal conductance but increased water use efficiency, and it decreased chlorophyll, betalain and polyphenol content in the leaves. It also decreased the foliar content of calcium, magnesium and potassium but not the iron and zinc content. The physiological parameters responded differently to sodium accumulation depending on the cultivar, suggesting a different relative importance of ionic and osmotic phases of salt stress among cultivars. Our results allowed us to identify the morpho-physiological traits of the cultivars with different salt tolerance levels.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:280777,

title = {Comparison between the impact of osmotic and NaCl treatments on the expression of genes coding for ion transporters in Oryza glaberrima Steud.},

author = {Hermann Prodjinoto and Willy Irakoze and Christophe Gandonou and Muriel Quinet and Stanley Lutts and Rupesh Kailasrao Deshmukh},

url = {http://hdl.handle.net/2078.1/280777},

doi = {10.1371/journal.pone.0290752},

issn = {1932-6203},

year  = {2023},

date = {2023-01-01},

journal = {PLoS One},

volume = {18},

number = {11},

pages = {e0290752},

publisher = {Public Library of Science},

abstract = {We analyzed the expression of genes coding for Na+ transporters (OsHKT1.5, OsHKT1.1, OsSOS1, OsSOS2, OsNHX1, OsNHX2), Cl- transporter (OsNRT1, OsCLC, OsCCC1) and gene coding for the transcription factor DREB (OsDREB2) involved in response to desiccation in two cultivars of O. glaberrrima differing in salt-resistance (salt-tolerant cultivar (TOG5307) and salt-sensitive (TOG 5949)) exposed to NaCl, PEG or both agents present simultaneously. Seedlings were grown in iso-osmotic nutrient solution (Ψs = -0.47±0.02 MPa) containing PEG 6,000 12.9% (water stress), NaCl 75 mM (salt stress) and PEG 6.4% + NaCl 37.5 mM (MIX-treatment) during 1 and 7 days. Plants were analyzed for gene expression, mineral nutrients, and photosynthetic-related parameters. Na+ and Cl- accumulations in salt-treated plants were lower in roots and shoots of TOG5307 comparatively to TOG5949 while water content decreased in TOG5307. TOG5307 exhibited tolerance to water stress and maintained higher net photosynthesis and water use efficiency than TOG5949 in response to all treatments, but was less efficient for osmotic adjustment. Dehydration tolerance of TOG5307 involves a higher OsDREB2 expression. TOG5307 also exhibited a higher OsSOS1, OsSOS2, OsNHX1 and OsNHX2 expression than TOG5949 in response to salinity. OsHKT1.5 was slightly induced in the shoot. OsHKT1.1 was recorded in the shoots but remained undetectable in the roots. Chloride and sodium accumulations were strongly reduced in the shoots when PEG was present. Salinity resistance in Oryza glaberrima implies tolerance to dehydration as well as complementary strategies of Na+ exclusion through the SOS system and Na+ tolerance through vacuolar sequestration.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:278610,

title = {Comparative and population genomics of buckwheat species reveal key determinants of flavor and fertility},

author = {Kaixuan Zhang and Yuqi He and Xiang Lu and Yaliang Shi and Hui Zhao and Xiaobo Li and Jinlong Li and Yang Liu and Yinan Ouyang and Yu Tang and Muriel Quinet},

url = {http://hdl.handle.net/2078.1/278610},

doi = {10.1016/j.molp.2023.08.013},

issn = {1674-2052},

year  = {2023},

date = {2023-01-01},

journal = {Molecular Plant},

volume = {16},

number = {9},

pages = {1427-1444},

publisher = {Elsevier BV},

abstract = {Common buckwheat (Fagopyrum esculentum) is an ancient crop with a world-wide distribution. Due to its excellent nutritional quality and high economic and ecological value, common buckwheat is becoming increasingly important throughout the world. The availability of a high-quality reference genome sequence and population genomic data will accelerate the breeding of common buckwheat, but the high heterozygosity due to the outcrossing nature has greatly hindered the genome assembly. Here we report the assembly of a chromosome-scale high-quality reference genome of F. esculentum var. homotropicum, a homozygous self-pollinating variant of common buckwheat. Comparative genomics revealed that two cultivated buckwheat species, common buckwheat (F. esculentum) and Tartary buckwheat (F. tataricum), underwent metabolomic divergence and ecotype differentiation. The expansion of several gene families in common buckwheat, including FhFAR genes, is associated with its wider distribution than Tartary buckwheat. Copy number variation of genes involved in the metabolism of flavonoids is associated with the difference of rutin content between common and Tartary buckwheat. Furthermore, we present a comprehensive atlas of genomic variation based on whole-genome resequencing of 572 accessions of common buckwheat. Population and evolutionary genomics reveal genetic variation associated with environmental adaptability and floral development between Chinese and non-Chinese cultivated groups. Genome-wide association analyses of multi-year agronomic traits with the content of flavonoids revealed that Fh05G014970 is a potential major regulator of flowering period, a key agronomic trait controlling the yield of outcrossing crops, and that Fh06G015130 is a crucial gene underlying flavor-associated flavonoids. Intriguingly, we found that the gene translocation and sequence variation of FhS-ELF3 contribute to the homomorphic self-compatibility of common buckwheat. Collectively, our results elucidate the genetic basis of speciation, ecological adaptation, fertility, and unique flavor of common buckwheat, and provide new resources for future genomics-assisted breeding of this economically important crop.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Luyckx2023943,

title = {Silicon reduces zinc absorption and triggers oxidative tolerance processes without impacting growth in young plants of hemp (Cannabis sativa L.)},

author = {M. Luyckx and J. -F. Hausman and G. Guerriero and S. Lutts},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85145591561&doi=10.1007%2fs11356-022-21797-4&partnerID=40&md5=6f70c5f1d5b52a8e52ddc999f4988b00},

doi = {10.1007/s11356-022-21797-4},

year  = {2023},

date = {2023-01-01},

journal = {Environmental Science and Pollution Research},

volume = {30},

number = {1},

pages = {943-955},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Phan20231789,

title = {Nitrogen Use Efficiency of Rice Cultivars (Oryza sativa L.) Under Salt Stress and Low Nitrogen Conditions},

author = {N. T. H. Phan and A. Heymans and M. Bonnave and S. Lutts and C. V. Pham and P. Bertin},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85129899164&doi=10.1007%2fs00344-022-10660-y&partnerID=40&md5=12c4f88c5de308c521c214d4fdae1186},

doi = {10.1007/s00344-022-10660-y},

year  = {2023},

date = {2023-01-01},

journal = {Journal of Plant Growth Regulation},

volume = {42},

number = {3},

pages = {1789-1803},

note = {cited By 1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Prodjinoto2023,

title = {Impact of salinity on yield-related parameters in two contrasting cultivars of Oryza glaberrima Steud. in Benin},

author = {H. Prodjinoto and C. Gandonou and W. Irakoze and S. Lutts},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85150359946&doi=10.1017%2fS0014479723000030&partnerID=40&md5=3f3a521a8b54b12779125d94a6b80c75},

doi = {10.1017/S0014479723000030},

year  = {2023},

date = {2023-01-01},

journal = {Experimental Agriculture},

volume = {59},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Zhou2023,

title = {Effect of NaCl and EDDS on Heavy Metal Accumulation in Kosteletzkya pentacarpos in Polymetallic Polluted Soil},

author = {M. Zhou and Z. Kiamarsi and R. Han and M. Kafi and S. Lutts},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85156196315&doi=10.3390%2fplants12081656&partnerID=40&md5=049e6072dd161c9ebaac4169d218a434},

doi = {10.3390/plants12081656},

year  = {2023},

date = {2023-01-01},

journal = {Plants},

volume = {12},

number = {8},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:259217,

title = {The Halophyte Species Solanum chilense Dun. Maintains Its Reproduction despite Sodium Accumulation in Its Floral Organs},

author = {Servane Bigot and Paula Pongrac and Martin Šala and Johannes T. van Elteren and Juan-Pablo Martínez and Stanley Lutts and Muriel Quinet},

url = {http://hdl.handle.net/2078.1/259217},

doi = {10.3390/plants11050672},

issn = {2223-7747},

year  = {2022},

date = {2022-01-01},

journal = {Plants},

volume = {11},

number = {5},

pages = {672},

publisher = {MDPI AG},

abstract = {Salinity is a growing global concern that affects the yield of crop species, including tomato (Solanum lycopersicum). Its wild relative Solanum chilense was reported to have halophyte properties. We compared salt resistance of both species during the reproductive phase, with a special focus on sodium localization in the flowers. Plants were exposed to NaCl from the seedling stage. Salinity decreased the number of inflorescences in both species but the number of flowers per inflorescence and sepal length only in S. lycopersicum. External salt supply decreased the stamen length in S. chilense, and it was associated with a decrease in pollen production and an increase in pollen viability. Although the fruit set was not affected by salinity, fruit weight and size decreased in S. lycopersicum. Concentrations and localization of Na, K, Mg, and Ca differed in reproductive structures of both species. Inflorescences and fruits of S. chilense accumulated more Na than S. lycopersicum. Sodium was mainly located in male floral organs of S. chilense but in non-reproductive floral organs in S. lycopersicum. The expression of Na transporter genes differed in flowers of both species. Overall, our results indicated that S. chilense was more salt-resistant than S. lycopersicum during the reproductive phase and that differences could be partly related to dissimilarities in element distribution and transport in flowers.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:270256,

title = {Silicon reduces zinc absorption and triggers oxidative tolerance processes without impacting growth in young plants of hemp (Cannabis sativa L.)},

author = {Marie Luyckx and Jean-François Hausman and Gea Guerriero and Stanley Lutts},

url = {http://hdl.handle.net/2078.1/270256},

doi = {10.1007/s11356-022-21797-4},

issn = {1614-7499},

year  = {2022},

date = {2022-01-01},

journal = {Environmental Science and Pollution Research},

volume = {30},

number = {1},

pages = {943-955},

publisher = {Springer},

address = {Heidelberg},

abstract = {Hemp (Cannabis sativa L.) is a promising crop for non-food agricultural production on soils contaminated by moderate doses of heavy metals, while silicon, as a beneficial element, is frequently reported to improve stressed plant behavior. Using a hydroponic system, plants of Cannabis sativa (cv. Santhica 27) were exposed for 1 week to 100 µM Zn in the presence or absence of 2 mM Si. Zinc accumulated in all plant organs but was mainly sequestered in the roots. Additional Si reduced Zn absorption but had no impact on Zn translocation. Zn accumulation had a slight negative impact on leaf number, stem length, and chlorophyll content, and additional Si did not mitigate these symptoms. Exogenous Si reduced the Zn-induced membrane lipid peroxidation (assessed by malondialdehyde quantification) and increased the total antioxidant activities estimated by the FRAP index. In the absence of Si, leaf phytochelatin and total glutathione were the highest in Zn-treated plants and Si significantly decreased their concentrations.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:269473,

title = {Impact of Silicon and Heavy Metals on Hemp (Cannabis sativa L.) Bast Fibres Properties: An Industrial and Agricultural Perspective},

author = {Marie Luyckx and Mathilde Blanquet and Arnaud Isenborghs and Gea Guerriero and Géraldine Bidar and Christophe Waterlot and Francis Douay and Stanley Lutts},

url = {http://hdl.handle.net/2078.1/269473},

doi = {10.1007/s41742-022-00446-1},

issn = {2008-2304},

year  = {2022},

date = {2022-01-01},

journal = {International Journal of Environmental Research},

volume = {16},

number = {5},

pages = {14},

publisher = {Springer Science and Business Media LLC},

abstract = {Heavy metal (HM) pollution in agricultural fields reduces crop productivity and quality, and poses a risk for human health. On these areas, the cultivation of crops devoted to biomaterial and bioenergy production thus constitutes an attractive alternative to food crop production. To maintain the soil productivity and to obtain a biomass compatible with the requirements of industry, the use of the beneficial metalloid silicon, applied on leaves during plant growth, has been considered. A field study using Cannabis sativa L. (cv. Futura 75) was performed during 2 consecutive years on two sites (one soil parcel contaminated with Cd, Zn and Pb, and one taken as uncontaminated control) in the North of France. One half of the plants received weekly foliar spray of Si (2 mM). Photosynthesis-related parameters were analysed in situ, while mineral concentration and fibre properties were determined after 4 months of culture. Our results suggest that Si application improves and preserves the mechanical properties of fibres when plants are exposed to HM stress. Si was also shown to increase by 27% the Cd concentration in leaves and by 30% the Zn concentration in stems. Contaminated fibres and shives containing immobilized HM could be used for specific purposes such as inclusion in cement aglomerates.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:258281,

title = {Endogenous Polyamines and Ethylene Biosynthesis in Relation to Germination of Osmoprimed Brassica napus Seeds under Salt Stress},

author = {Katarzyna Lechowska and Łukasz Wojtyla and Muriel Quinet and Szymon Kubala and Stanley Lutts and Małgorzata Garnczarska},

url = {http://hdl.handle.net/2078.1/258281},

doi = {10.3390/ijms23010349},

issn = {1422-0067},

year  = {2022},

date = {2022-01-01},

journal = {International Journal of Molecular Sciences},

volume = {23},

number = {1},

pages = {349},

publisher = {MDPI AG},

abstract = {Currently, seed priming is reported as an efficient and low-cost approach to increase crop yield, which could not only promote seed germination and improve plant growth state but also increase abiotic stress tolerance. Salinity represents one of the most significant abiotic stresses that alters multiple processes in plants. The accumulation of polyamines (PAs) in response to salt stress is one of the most remarkable plant metabolic responses. This paper examined the effect of osmopriming on endogenous polyamine metabolism at the germination and early seedling development of Brassica napus in relation to salinity tolerance. Free, conjugated and bound polyamines were analyzed, and changes in their accumulation were discussed with literature data. The most remarkable differences between the corresponding osmoprimed and unprimed seeds were visible in the free (spermine) and conjugated (putrescine, spermidine) fractions. The arginine decarboxylase pathway seems to be responsible for the accumulation of PAs in primed seeds. The obvious impact of seed priming on tyramine accumulation was also demonstrated. Moreover, the level of ethylene increased considerably in seedlings issued from primed seeds exposed to salt stress. It can be concluded that the polyamines are involved in creating the beneficial effect of osmopriming on germination and early growth of Brassica napus seedlings under saline conditions through moderate changes in their biosynthesis and accumulation.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:269465,

title = {Effects of a Local Tomato Rootstock on the Agronomic, Functional and Sensory Quality of the Fruit of a Recovered Local Tomato (Solanum lycopersicum L.) Named “Tomate Limachino Antiguo”},

author = {Juan Pablo Martínez and Raúl Fuentes and Karen Farías and Nelson Loyola and Alejandra Freixas and Claudia Stange and Boris Sagredo and Muriel Quinet and Stanley Lutts},

url = {http://hdl.handle.net/2078.1/269465},

doi = {10.3390/agronomy12092178},

issn = {2073-4395},

year  = {2022},

date = {2022-01-01},

journal = {Agronomy},

volume = {12},

number = {9},

pages = {2178},

publisher = {MDPI AG},

abstract = {The Old Limachino Tomato is a valuable fruit with exceptional nutritional values and organoleptic sensory properties. However, it suffers from a short shelf-life, compromising post-harvest behavior. As an attempt to improve the fruit’s qualities, Limachino (L) scion was grafted onto rootstock from the rustic landrace Poncho Negro (R). Fruits produced in this graft combination were compared with fruits produced by self-grafted plants (L/L) and from a long-shelf-life cultivar Seminis (LSL). The trials were carried out for 146 days during summer of two consecutive years. Poncho Negro rootstock increased the total number of fruits produced by Limachino scion (L/R). It did not affect the fresh weight of individual fruits but reduced their water content. It has no impact on the Limachino fruit form (quality), a typical characteristic well appreciated by consumers. Fruits produced by LSL exhibited a higher firmness but a lower titratable acidity and antioxidant capacity than L/R and L/L fruits. Panels of 104 untrained final consumers and a trained panel of 13 experts attributed the highest value to L/R fruits and the lowest one to LSL. It was concluded that Poncho Negro rootstock contributes to increasing preferences and the level of acceptability towards Limachino fruits. Further research is needed to develop local technologies in order to expand the production of local tomatoes that are highly valued by consumers.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boreal:258278,

title = {Differential effects of sulfate and chloride salinities on rice (Oryza sativa L.) gene expression patterns: a comparative transcriptomic and physiological approach},

author = {Willy Irakoze and Muriel Quinet and Hermann Prodjinoto and Gervais Rufyikiri and Séverin Nijimbere and Stanley Lutts},

url = {http://hdl.handle.net/2078.1/258278},

doi = {10.1016/j.cpb.2022.100237},

issn = {2214-6628},

year  = {2022},

date = {2022-01-01},

journal = {Current Plant Biology},

pages = {100237},

publisher = {Elsevier BV},

address = {Amsterdam},

abstract = {Salinity is a challenge to rice production but most studies are dealing with NaCl and rarely consider Na2SO4 despite its importance in numerous areas of the world. To elucidate genome-level responses to chloride or sulfate salinity stress, seedlings from rice cv. IKP have been exposed for 48 h to two types of Na+-isostrenght nutrient solution (NaCl 200 mM (EC18.84 dS m-1; Ψs = -0.983 MPa); and Na2SO4 100 mM (EC18.05 dS m-1; Ψs = -0.838 MPa)). A combined transcriptomic (microarray analysis) and physiological study was performed. NaCl was more toxic than Na2SO4 to rice seedlings. Contrasting genes were expressed under sulfate and chloride salinity, the difference being the most remarkable in root. Most of the genes involved in response to salt stress were up-regulated in Na2SO4-treated plants while more genes were down-regulated in NaCl-treated plants. Proline accumulated to a higher extent in NaCl-treated plants in relation to up-regulation of genes coding for Δ1-pyrroline-5-carboxylate synthetase and Ornithine-δ-aminotransferase which induced a higher activity of these enzymes in plants exposed to NaCl compared to Na2SO4. In contrast, sucrose accumulated in Na2SO4-treated plants while reducing sugars accumulated in NaCl-exposed ones. These differences could be explained by activities of sucrose-phosphate-synthase, sucrose synthase and acidic invertase but not by upregulation of the corresponding genes. Regulations of the expression of genes coding for signal sensing, perception and transduction and for transcription factors were completely different in response to NaCl and Na2SO4 suggesting that the nature of the counter anion is of primary importance in stress perception and plant response.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kiamarsi2022483,

title = {Evaluating the bio-removal of crude oil by vetiver grass (Vetiveria zizanioides L.) in interaction with bacterial consortium exposed to contaminated artificial soils},

author = {Z. Kiamarsi and M. Kafi and M. Soleimani and A. Nezami and S. Lutts},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85111922477&doi=10.1080%2f15226514.2021.1954876&partnerID=40&md5=b84cae1ffa4b1dd2b2e2b07eeb4c9a1b},

doi = {10.1080/15226514.2021.1954876},

year  = {2022},

date = {2022-01-01},

journal = {International Journal of Phytoremediation},

volume = {24},

number = {5},

pages = {483-492},

note = {cited By 3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Luyckx2022,

title = {Impact of Silicon and Heavy Metals on Hemp (Cannabis sativa L.) Bast Fibres Properties: An Industrial and Agricultural Perspective},

author = {M. Luyckx and M. Blanquet and A. Isenborghs and G. Guerriero and G. Bidar and C. Waterlot and F. Douay and S. Lutts},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85137603886&doi=10.1007%2fs41742-022-00446-1&partnerID=40&md5=e156b23f019ad99894b514329256d0dc},

doi = {10.1007/s41742-022-00446-1},

year  = {2022},

date = {2022-01-01},

journal = {International Journal of Environmental Research},

volume = {16},

number = {5},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}